1L1G

The Structure of Porcine Pancreatic Elastase Complexed with Xenon and Bromide, Cryoprotected with Glycerol

Summary for 1L1G

Entry DOI	10.2210/pdb1l1g/pdb
Descriptor	ELASTASE 1, SULFATE ION, SODIUM ION, ... (7 entities in total)
Functional Keywords	xenon, bromide, ppe, hydrolase
Biological source	Sus scrofa (pig)
Cellular location	Secreted: P00772
Total number of polymer chains	1
Total formula weight	26910.69
Authors	Panjikar, S.,Tucker, P.A. (deposition date: 2002-02-16, release date: 2002-08-28, Last modification date: 2024-11-06)
Primary citation	Panjikar, S.,Tucker, P.A. Xenon derivatization of halide-soaked protein crystals. Acta Crystallogr.,Sect.D, 58:1413-1420, 2002 Cited by PubMed Abstract: Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined. PubMed: 12198297 DOI: 10.1107/S0907444902011010 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.5 Å)

Structure validation