1L0Z

THE STRUCTURE OF PORCINE PANCREATIC ELASTASE COMPLEXED WITH XENON AND BROMIDE, CRYOPROTECTED WITH DRY PARAFFIN OIL

1L0Z の概要

• エントリーDOI: 10.2210/pdb1l0z/pdb
• 分子名称: ELASTASE 1, SODIUM ION, BROMIDE ION, ... (5 entities in total)
• 機能のキーワード: xenon, bromide, ppe, hydrolase
• 由来する生物種: Sus scrofa (pig)
• 細胞内の位置: Secreted: P00772
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28640.23

構造登録者

Tucker, P.A.,Panjikar, S. (登録日: 2002-02-14, 公開日: 2002-08-28, 最終更新日: 2024-10-30)

主引用文献

Panjikar, S.,Tucker, P.A.
Xenon derivatization of halide-soaked protein crystals.
Acta Crystallogr.,Sect.D, 58:1413-1420, 2002

PubMed Abstract: Crystals of porcine pancreatic elastase can be derived by soaking in high-molarity bromide solutions. These crystals, either in a glycerol-based cryoprotectant solution or in paraffin oil, can be subsequently pressurized under a xenon atmosphere to incorporate xenon. When paraffin oil is used, a larger number of bromide ions are observed on the protein surface. Intensity data collected to lower energy than the bromine absorption edge can be used to determine the xenon position and the resultant phase information can be used to determine the bromine substructure from data collected to higher energy than the bromine absorption edge. The method would appear to have general applicability where large substructures need to be determined.

PubMed: 12198297
DOI: 10.1107/S0907444902011010

実験手法

X-RAY DIFFRACTION (1.5 Å)