1KVY

CARBOXYLIC ESTER HYDROLASE, SINGLE MUTANT D49E COORDINATED TO CALCIUM

1KVY の概要

• エントリーDOI: 10.2210/pdb1kvy/pdb
• 分子名称: PHOSPHOLIPASE A2, CALCIUM ION (3 entities in total)
• 機能のキーワード: hydrolase, enzyme, carboxylic ester hydrolase, mutant
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P00593
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13864.61

構造登録者

Sundaralingam, M. (登録日: 1998-04-29, 公開日: 1998-11-18, 最終更新日: 2024-10-23)

主引用文献

Sekar, K.,Biswas, R.,Li, Y.,Tsai, M.,Sundaralingam, M.
Structures of the catalytic site mutants D99A and H48Q and the calcium-loop mutant D49E of phospholipase A2.
Acta Crystallogr.,Sect.D, 55:443-447, 1999

PubMed Abstract: Crystal structures of the active-site mutants D99A and H48Q and the calcium-loop mutant D49E of bovine phospholipase A2 have been determined at around 1.9 A resolution. The D99A mutant is isomorphous to the orthorhombic recombinant enzyme, space group P212121. The H48Q and the calcium-loop mutant D49E are isomorphous to the trigonal recombinant enzyme, space group P3121. The two active-site mutants show no major structural perturbations. The structural water is absent in D99A and, therefore, the hydrogen-bonding scheme is changed. In H48Q, the catalytic water is present and hydrogen bonded to Gln48 N, but the second water found in native His48 is absent. In the calcium-loop mutant D49E, the two water molecules forming the pentagonal bipyramid around calcium are absent and only one O atom of the Glu49 carboxylate group is coordinated to calcium, resulting in only four ligands.

PubMed: 10089353
DOI: 10.1107/S0907444998013699

実験手法

X-RAY DIFFRACTION (1.9 Å)