1KUJ

Crystal structure of Jacalin complexed with 1-O-methyl-alpha-D-mannose

1KUJ の概要

• エントリーDOI: 10.2210/pdb1kuj/pdb
• 関連するPDBエントリー: 1jac 1jot 1ku8
• 分子名称: JACALIN ALPHA CHAIN, JACALIN BETA CHAIN, methyl alpha-D-mannopyranoside, ... (4 entities in total)
• 機能のキーワード: lectin, beta-prism fold, carbohydrate binding, sugar binding protein
• 由来する生物種: Artocarpus integer; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 66958.90

構造登録者

Bourne, Y.,Astoul, C.H.,Zamboni, V.,Peumans, W.J.,Menu-Bouaouiche, L.,Van Damme, E.J.M.,Barre, A.,Rouge, P. (登録日: 2002-01-22, 公開日: 2002-06-19, 最終更新日: 2023-08-16)

主引用文献

Bourne, Y.,Astoul, C.H.,Zamboni, V.,Peumans, W.J.,Menu-Bouaouiche, L.,Van Damme, E.J.,Barre, A.,Rouge, P.
Structural basis for the unusual carbohydrate-binding specificity of jacalin towards galactose and mannose.
Biochem.J., 364:173-180, 2002

PubMed Abstract: Evidence is presented that the specificity of jacalin, the seed lectin from jack fruit (Artocarpus integrifolia), is not directed exclusively against the T-antigen disaccharide Galbeta1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin-alpha-methyl-mannose complex at 2 A resolution, demonstrated clearly that jacalin is fully capable of binding mannose. Besides mannose, jacalin also interacts readily with glucose, N-acetylneuraminic acid and N-acetylmuramic acid. Structural analyses demonstrated that the relatively large size of the carbohydrate-binding site enables jacalin to accommodate monosaccharides with different hydroxyl conformations and provided unambiguous evidence that the beta-prism structure of jacalin is a sufficiently flexible structural scaffold to confer different carbohydrate-binding specificities to a single lectin.

PubMed: 11988090

実験手法

X-RAY DIFFRACTION (2 Å)