1KSX

Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex

1KSX の概要

• エントリーDOI: 10.2210/pdb1ksx/pdb
• 関連するPDBエントリー: 1F08 1KSY
• 分子名称: E1 Recognition Sequence, REPLICATION PROTEIN E1 (3 entities in total)
• 機能のキーワード: papillomavirus, dna-binding domain, replication, initiator protein, helicase, replication-dna complex, replication/dna
• 由来する生物種: Bovine papillomavirus
• 細胞内の位置: Host nucleus: P03116
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 160799.05

構造登録者

Enemark, E.J.,Stenlund, A.,Joshua-Tor, L. (登録日: 2002-01-14, 公開日: 2002-03-15, 最終更新日: 2023-08-16)

主引用文献

Enemark, E.J.,Stenlund, A.,Joshua-Tor, L.
Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex.
EMBO J., 21:1487-1496, 2002

PubMed Abstract: Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.

PubMed: 11889054
DOI: 10.1093/emboj/21.6.1487

実験手法

X-RAY DIFFRACTION (3.2 Å)