1KQ3

CRYSTAL STRUCTURE OF A GLYCEROL DEHYDROGENASE (TM0423) FROM THERMOTOGA MARITIMA AT 1.5 A RESOLUTION

Summary for 1KQ3

• Entry DOI: 10.2210/pdb1kq3/pdb
• Related: 1JPU 1JQ5 1JQA
• Descriptor: glycerol dehydrogenase, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: glycerol dehydrogenase, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi, oxidoreductase
• Biological source: Thermotoga maritima
• Total number of polymer chains: 1
• Total formula weight: 41421.08

Authors

Wilson, I.A.,Miller, M.D.,Joint Center for Structural Genomics (JCSG) (deposition date: 2002-01-03, release date: 2002-02-27, Last modification date: 2024-02-14)

Primary citation

Lesley, S.A.,Kuhn, P.,Godzik, A.,Deacon, A.M.,Mathews, I.,Kreusch, A.,Spraggon, G.,Klock, H.E.,McMullan, D.,Shin, T.,Vincent, J.,Robb, A.,Brinen, L.S.,Miller, M.D.,McPhillips, T.M.,Miller, M.A.,Scheibe, D.,Canaves, J.M.,Guda, C.,Jaroszewski, L.,Selby, T.L.,Elsliger, M.-A.,Wooley, J.,Taylor, S.S.,Hodgson, K.O.,Wilson, I.A.,Schultz, P.G.,Stevens, R.C.
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
Proc.Natl.Acad.Sci.USA, 99:11664-11669, 2002

PubMed Abstract: Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

PubMed: 12193646
DOI: 10.1073/pnas.142413399

Experimental method

X-RAY DIFFRACTION (1.5 Å)