1KQ3

CRYSTAL STRUCTURE OF A GLYCEROL DEHYDROGENASE (TM0423) FROM THERMOTOGA MARITIMA AT 1.5 A RESOLUTION

1KQ3 の概要

• エントリーDOI: 10.2210/pdb1kq3/pdb
• 関連するPDBエントリー: 1JPU 1JQ5 1JQA
• 分子名称: glycerol dehydrogenase, ZINC ION, CHLORIDE ION, ... (5 entities in total)
• 機能のキーワード: glycerol dehydrogenase, structural genomics, joint center for structural genomics, jcsg, protein structure initiative, psi, oxidoreductase
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41421.08

構造登録者

Wilson, I.A.,Miller, M.D.,Joint Center for Structural Genomics (JCSG) (登録日: 2002-01-03, 公開日: 2002-02-27, 最終更新日: 2024-02-14)

主引用文献

Lesley, S.A.,Kuhn, P.,Godzik, A.,Deacon, A.M.,Mathews, I.,Kreusch, A.,Spraggon, G.,Klock, H.E.,McMullan, D.,Shin, T.,Vincent, J.,Robb, A.,Brinen, L.S.,Miller, M.D.,McPhillips, T.M.,Miller, M.A.,Scheibe, D.,Canaves, J.M.,Guda, C.,Jaroszewski, L.,Selby, T.L.,Elsliger, M.-A.,Wooley, J.,Taylor, S.S.,Hodgson, K.O.,Wilson, I.A.,Schultz, P.G.,Stevens, R.C.
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
Proc.Natl.Acad.Sci.USA, 99:11664-11669, 2002

PubMed Abstract: Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

PubMed: 12193646
DOI: 10.1073/pnas.142413399

実験手法

X-RAY DIFFRACTION (1.5 Å)