1KP7
Conserved RNA Structure within the HCV IRES eIF3 Binding Site
Summary for 1KP7
| Entry DOI | 10.2210/pdb1kp7/pdb |
| Descriptor | Hepatitis C Virus Internal Ribosome Entry Site Fragment (1 entity in total) |
| Functional Keywords | cytosine mismatch, eif3, hcv, internal loop, ires, s-turn, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 9595.75 |
| Authors | Gallego, J.,Klinck, R.,Collier, A.J.,Cole, P.T.,Harris, S.J.,Harrison, G.P.,Aboul-ela, F.,Walker, S.,Varani, G. (deposition date: 2001-12-29, release date: 2002-04-10, Last modification date: 2024-05-22) |
| Primary citation | Collier, A.J.,Gallego, J.,Klinck, R.,Cole, P.T.,Harris, S.J.,Harrison, G.P.,Aboul-Ela, F.,Varani, G.,Walker, S. A conserved RNA structure within the HCV IRES eIF3-binding site. Nat.Struct.Biol., 9:375-380, 2002 Cited by PubMed Abstract: The hepatitis C virus (HCV) internal ribosome entry site (IRES) is recognized specifically by the small ribosomal subunit and eukaryotic initiation factor 3 (eIF3) before viral translation initiation. Using extensive mutagenesis and structure probing analysis, we show that the eIF3-binding domain of the HCV IRES contains an internal loop structure (loop IIIb) and an adjacent mismatched helix that are important for IRES-dependent initiation of translation. NMR studies reveal a unique three-dimensional structure for this internal loop that is conserved between viral isolates of varying primary sequence in this region. These data indicate that internal loop IIIb may be an attractive target for structure-based design of new antiviral agents. PubMed: 11927954PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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