1KNV

Bse634I restriction endonuclease

1KNV の概要

• エントリーDOI: 10.2210/pdb1knv/pdb
• 関連するPDBエントリー: 1cfr
• 分子名称: Bse634I restriction endonuclease, ACETATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: restriction endonuclease, apo-enzyme, hydrolase
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67993.10

構造登録者

Grazulis, S.,Deibert, M.,Rimseliene, R.,Skirgaila, R.,Sasnauskas, G.,Lagunavicius, A.,Repin, V.,Urbanke, C.,Huber, R.,Siksnys, V. (登録日: 2001-12-19, 公開日: 2002-02-27, 最終更新日: 2024-03-13)

主引用文献

Grazulis, S.,Deibert, M.,Rimseliene, R.,Skirgaila, R.,Sasnauskas, G.,Lagunavicius, A.,Repin, V.,Urbanke, C.,Huber, R.,Siksnys, V.
Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.
Nucleic Acids Res., 30:876-885, 2002

PubMed Abstract: Crystal structures of Type II restriction endonucleases demonstrate a conserved common core and active site residues but diverse structural elements involved in DNA sequence discrimination. Comparative structural analysis of restriction enzymes recognizing the same nucleotide sequence might therefore contribute to our understanding of the structural diversity of specificity determinants within restriction enzymes. We have solved the crystal structure of the Bacillus stearothermophilus restriction endonuclease Bse634I by the multiple isomorphous replacement technique to 2.17 A resolution. Bse634I is an isoschisomer of the Cfr10I restriction enzyme whose crystal structure has been reported previously. Comparative structural analysis of the first pair of isoschisomeric enzymes revealed conserved structural determinants of sequence recognition and catalysis. However, conformations of the N-terminal subdomains differed between Bse634I/Cfr10I, suggesting a rigid body movement that might couple DNA recognition and catalysis. Structural similarities extend to the quaternary structure level: crystal contacts suggest that Bse634I similarly to Cfr10I is arranged as a tetramer. Kinetic analysis reveals that Bse634I is able to interact simultaneously with two recognition sites supporting the tetrameric architecture of the protein. Thus, restriction enzymes Bse634I, Cfr10I and NgoMIV, recognizing overlapping nucleotide sequences, exhibit a conserved tetrameric architecture that is of functional importance.

PubMed: 11842098
DOI: 10.1093/nar/30.4.876

実験手法

X-RAY DIFFRACTION (2.17 Å)