1KN2

CATALYTIC ANTIBODY D2.3 COMPLEX

Summary for 1KN2

• Entry DOI: 10.2210/pdb1kn2/pdb
• Related: 1KN4 1YEC 1YEF 1YEG 1YEH 1YEI 1YEJ 1YEK
• Descriptor: IG ANTIBODY D2.3 (LIGHT CHAIN), IG ANTIBODY D2.3 (HEAVY CHAIN), ZINC ION, ... (5 entities in total)
• Functional Keywords: abzyme, transition state analog, immune system
• Biological source: Mus musculus (house mouse); More
• Total number of polymer chains: 2
• Total formula weight: 49027.18

Authors

Gigant, B.,Knossow, M. (deposition date: 2001-12-18, release date: 2002-03-13, Last modification date: 2024-10-09)

Primary citation

D'Souza, L.J.,Gigant, B.,Knossow, M.,Green, B.S.
Remarkable remote chiral recognition in a reaction mediated by a catalytic antibody.
J.Am.Chem.Soc., 124:2114-2115, 2002

PubMed Abstract: The crystal structures of catalytic antibody D2.3 Fab with the two enantiomers, 7D and 7L, which represent transition state analogues for the hydrolysis of the corresponding esters, 6D and 6L, were determined to better understand remarkable reactivity differences: the L-ester displayed significantly tighter binding (K(M)) and increased catalytic activity (k(cat)) with D2.3, even though the chiral center is 7 bonds distant from the reaction center. Surprisingly, the electron densities of the liganded phosphonates, 7D and 7L, within the D2.3 binding/reaction site were essentially identical, highlighting the subtle influences of protein interactions on chemical behavior.

PubMed: 11878955
DOI: 10.1021/ja0170504

Experimental method

X-RAY DIFFRACTION (1.9 Å)