1KN2

CATALYTIC ANTIBODY D2.3 COMPLEX

1KN2 の概要

• エントリーDOI: 10.2210/pdb1kn2/pdb
• 関連するPDBエントリー: 1KN4 1YEC 1YEF 1YEG 1YEH 1YEI 1YEJ 1YEK
• 分子名称: IG ANTIBODY D2.3 (LIGHT CHAIN), IG ANTIBODY D2.3 (HEAVY CHAIN), ZINC ION, ... (5 entities in total)
• 機能のキーワード: abzyme, transition state analog, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49027.18

構造登録者

Gigant, B.,Knossow, M. (登録日: 2001-12-18, 公開日: 2002-03-13, 最終更新日: 2024-10-09)

主引用文献

D'Souza, L.J.,Gigant, B.,Knossow, M.,Green, B.S.
Remarkable remote chiral recognition in a reaction mediated by a catalytic antibody.
J.Am.Chem.Soc., 124:2114-2115, 2002

PubMed Abstract: The crystal structures of catalytic antibody D2.3 Fab with the two enantiomers, 7D and 7L, which represent transition state analogues for the hydrolysis of the corresponding esters, 6D and 6L, were determined to better understand remarkable reactivity differences: the L-ester displayed significantly tighter binding (K(M)) and increased catalytic activity (k(cat)) with D2.3, even though the chiral center is 7 bonds distant from the reaction center. Surprisingly, the electron densities of the liganded phosphonates, 7D and 7L, within the D2.3 binding/reaction site were essentially identical, highlighting the subtle influences of protein interactions on chemical behavior.

PubMed: 11878955
DOI: 10.1021/ja0170504

実験手法

X-RAY DIFFRACTION (1.9 Å)