1KMN

HISTIDYL-TRNA SYNTHETASE COMPLEXED WITH HISTIDINOL AND ATP

Summary for 1KMN

• Entry DOI: 10.2210/pdb1kmn/pdb
• Descriptor: HISTIDYL-TRNA SYNTHETASE, L-histidinol, ADENOSINE-5'-TRIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: aminoacyl-trna synthase, ligase, synthetase
• Biological source: Escherichia coli
• Cellular location: Cytoplasm: P60906
• Total number of polymer chains: 4
• Total formula weight: 190938.70

Authors

Arnez, J.G.,Francklyn, C.S.,Moras, D. (deposition date: 1997-05-09, release date: 1997-12-17, Last modification date: 2023-11-15)

Primary citation

Arnez, J.G.,Augustine, J.G.,Moras, D.,Francklyn, C.S.
The first step of aminoacylation at the atomic level in histidyl-tRNA synthetase.
Proc.Natl.Acad.Sci.USA, 94:7144-7149, 1997

PubMed Abstract: The crystal structure of an enzyme-substrate complex with histidyl-tRNA synthetase from Escherichia coli, ATP, and the amino acid analog histidinol is described and compared with the previously obtained enzyme-product complex with histidyl-adenylate. An active site arginine, Arg-259, unique to all histidyl-tRNA synthetases, plays the role of the catalytic magnesium ion seen in seryl-tRNA synthetase. When Arg-259 is substituted with histidine, the apparent second order rate constant (kcat/Km) for the pyrophosphate exchange reaction and the aminoacylation reaction decreases 1,000-fold and 500-fold, respectively. Crystals soaked with MnCl2 reveal the existence of two metal binding sites between beta- and gamma-phosphates; these sites appear to stabilize the conformation of the pyrophosphate. The use of both conserved metal ions and arginine in phosphoryl transfer provides evidence of significant early functional divergence of class II aminoacyl-tRNA synthetases.

PubMed: 9207058
DOI: 10.1073/pnas.94.14.7144

Experimental method

X-RAY DIFFRACTION (2.8 Å)