1KLP

The Solution Structure of Acyl Carrier Protein from Mycobacterium tuberculosis

1KLP の概要

• エントリーDOI: 10.2210/pdb1klp/pdb
• 分子名称: MEROMYCOLATE EXTENSION ACYL CARRIER PROTEIN (1 entity in total)
• 機能のキーワード: four-helix bundle, ligand transport
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm (By similarity): P0A4W6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12532.87

構造登録者

Wong, H.C.,Liu, G.,Zhang, Y.-M.,Rock, C.O.,Zheng, J. (登録日: 2001-12-12, 公開日: 2002-06-07, 最終更新日: 2024-05-01)

主引用文献

Wong, H.C.,Liu, G.,Zhang, Y.M.,Rock, C.O.,Zheng, J.
The solution structure of acyl carrier protein from Mycobacterium tuberculosis.
J.Biol.Chem., 277:15874-15880, 2002

PubMed Abstract: Acyl carrier protein (ACP) performs the essential function of shuttling the intermediates between the enzymes that constitute the type II fatty acid synthase system. Mycobacterium tuberculosis is unique in producing extremely long mycolic acids, and tubercular ACP, AcpM, is also unique in possessing a longer carboxyl terminus than other ACPs. We determined the solution structure of AcpM using protein NMR spectroscopy to define the similarities and differences between AcpM and the typical structures. The amino-terminal region of the structure is well defined and consists of four helices arranged in a right-handed bundle held together by interhelical hydrophobic interactions similar to the structures of other bacterial ACPs. The unique carboxyl-terminal extension from helix IV has a "melted down" feature, and the end of the molecule is a random coil. A comparison of the apo- and holo-forms of AcpM revealed that the 4'-phosphopantetheine group oscillates between two states; in one it is bound to a hydrophobic groove on the surface of AcpM, and in another it is solvent-exposed. The similarity between AcpM and other ACPs reveals the conserved structural motif that is recognized by all type II enzymes. However, the function of the coil domain extending from helix IV to the carboxyl terminus remains enigmatic, but its structural characteristics suggest that it may interact with the very long chain intermediates in mycolic acid biosynthesis or control specific protein-protein interactions.

PubMed: 11825906
DOI: 10.1074/jbc.M112300200

実験手法

SOLUTION NMR