1KHF

PEPCK complex with PEP

1KHF の概要

• エントリーDOI: 10.2210/pdb1khf/pdb
• 関連するPDBエントリー: 1KHB 1KHE 1KHG
• 分子名称: Phosphoenolpyruvate Carboxykinase, cytosolic (GTP), MANGANESE (II) ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: gluconeogenesis, p-loop, lyase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P35558
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 69899.81

構造登録者

Dunten, P.,Belunis, C.,Crowther, R.,Hollfelder, K.,Kammlott, U.,Levin, W.,Michel, H.,Ramsey, G.B.,Swain, A.,Weber, D.,Wertheimer, S.J. (登録日: 2001-11-29, 公開日: 2002-02-27, 最終更新日: 2024-02-14)

主引用文献

Dunten, P.,Belunis, C.,Crowther, R.,Hollfelder, K.,Kammlott, U.,Levin, W.,Michel, H.,Ramsey, G.B.,Swain, A.,Weber, D.,Wertheimer, S.J.
Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site.
J.Mol.Biol., 316:257-264, 2002

PubMed Abstract: We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates. These structures are the first to be determined for a GTP-dependent PEPCK, and provide the first view of a novel GTP-binding site unique to the GTP-dependent PEPCK family. Three phenylalanine residues form the walls of the guanine-binding pocket on the enzyme's surface and, most surprisingly, one of the phenylalanine side-chains contributes to the enzyme's specificity for GTP. PEPCK catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Because the gluconeogenic pathway contributes to the fasting hyperglycemia of type II diabetes, inhibitors of PEPCK may be useful in the treatment of diabetes.

PubMed: 11851336
DOI: 10.1006/jmbi.2001.5364

実験手法

X-RAY DIFFRACTION (2.02 Å)