1KHC

Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b

1KHC の概要

• エントリーDOI: 10.2210/pdb1khc/pdb
• 分子名称: DNA cytosine-5 methyltransferase 3B2, UNKNOWN ATOM OR ION (3 entities in total)
• 機能のキーワード: five beta-sheets barrel followed by five-helix bundle, transferase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: O88509
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16670.04

構造登録者

Qiu, C.,Sawada, K.,Zhang, X.,Cheng, X. (登録日: 2001-11-29, 公開日: 2002-02-27, 最終更新日: 2024-02-14)

主引用文献

Qiu, C.,Sawada, K.,Zhang, X.,Cheng, X.
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds.
Nat.Struct.Biol., 9:217-224, 2002

PubMed Abstract: The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.

PubMed: 11836534

実験手法

X-RAY DIFFRACTION (1.8 Å)