1KEJ

Crystal Structure of Murine Terminal Deoxynucleotidyl Transferase complexed with ddATP

1KEJ の概要

• エントリーDOI: 10.2210/pdb1kej/pdb
• 関連するPDBエントリー: 1BPY 1JMS 1KDH
• 分子名称: Terminal deoxynucleotidyltransferase short isoform, COBALT (II) ION, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: polymerase, nucleotidyl transferase, pol x family, transferase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Isoform TDT-S: Nucleus. Isoform TDT-L: Cytoplasm: P09838
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 42531.87

構造登録者

Delarue, M.,Boule, J.B.,Lescar, J.,Expert-Bezancon, N.,Jourdan, N.,Sukumar, N.,Rougeon, F.,Papanicolaou, C. (登録日: 2001-11-16, 公開日: 2002-05-16, 最終更新日: 2023-08-16)

主引用文献

Delarue, M.,Boule, J.B.,Lescar, J.,Expert-Bezancon, N.,Jourdan, N.,Sukumar, N.,Rougeon, F.,Papanicolaou, C.
Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase.
EMBO J., 21:427-439, 2002

PubMed Abstract: The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 A resolution reveals a typical DNA polymerase beta-like fold locked in a closed form. In addition, the structures of two different binary complexes, one with an oligonucleotide primer and the other with an incoming ddATP-Co(2+) complex, show that the substrates and the two divalent ions in the catalytic site are positioned in TdT in a manner similar to that described for the human DNA polymerase beta ternary complex, suggesting a common two metal ions mechanism of nucleotidyl transfer in these two proteins. The inability of TdT to accommodate a template strand can be explained by steric hindrance at the catalytic site caused by a long lariat-like loop, which is absent in DNA polymerase beta. However, displacement of this discriminating loop would be sufficient to unmask a number of evolutionarily conserved residues, which could then interact with a template DNA strand. The present structure can be used to model the recently discovered human polymerase mu, with which it shares 43% sequence identity.

PubMed: 11823435
DOI: 10.1093/emboj/21.3.427

実験手法

X-RAY DIFFRACTION (3 Å)