1KD5

The Crystal Structure of r(GGUCACAGCCC)2 metal free form

1KD5 の概要

• エントリーDOI: 10.2210/pdb1kd5/pdb
• 分子名称: 5'-R(*GP*GP*UP*CP*AP*CP*AP*GP*CP*CP*C)-3' (2 entities in total)
• 機能のキーワード: rna duplex, rna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6962.29

構造登録者

Kacer, V.,Scaringe, S.A.,Scarsdale, J.N.,Rife, J.P. (登録日: 2001-11-12, 公開日: 2003-03-04, 最終更新日: 2024-02-07)

主引用文献

Kacer, V.,Scaringe, S.A.,Scarsdale, J.N.,Rife, J.P.
Crystal structures of r(GGUCACAGCCC)2.
Acta Crystallogr.,Sect.D, 59:423-432, 2003

PubMed Abstract: Crystals of small RNAs, which regularly diffract to very high resolution, can often be readily obtained. Unfortunately, for some RNAs the conformations adopted in the crystalline form are different from those found in solution. For example, short RNAs that form hairpins in solution virtually never crystallize thus; rather, they form duplexes. Nevertheless, these unintended structures have contributed greatly to the understanding of RNA structure. In a similar occurrence, the homodimer r(GGUCACAGCCC)(2) has been crystallized from an 11-mer/12-mer heteroduplex, r(GGCUGAAGUCCG)/r(GGUCACAGCCC). This surprising phenomenon was observed under a variety of crystallization conditions. The structure of the homoduplex was determined from crystals that differed in the precipitant used and the type of metal present. In all cases, the resulting homoduplexes contain ten base pairings, of which the central six are non-canonical pairings. In two of the variants, ordered metal-binding sites were observed: two equivalent octacoordinate Tl(+) sites in one and two equivalent nanocoordinate Ba(2+) sites in another.

PubMed: 12595698
DOI: 10.1107/S0907444902021911

実験手法

X-RAY DIFFRACTION (1.58 Å)