1KCB

Crystal Structure of a NO-forming Nitrite Reductase Mutant: an Analog of a Transition State in Enzymatic Reaction

1KCB の概要

• エントリーDOI: 10.2210/pdb1kcb/pdb
• 分子名称: Nitrite Reductase, COPPER (II) ION (3 entities in total)
• 機能のキーワード: copper-containing nitrite reductase, beta barrel, oxidoreductase
• 由来する生物種: Achromobacter cycloclastes
• 細胞内の位置: Periplasm: P25006
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37202.86

構造登録者

Liu, S.Q.,Chang, T.,Liu, M.Y.,LeGall, J.,Chang, W.C.,Zhang, J.P.,Liang, D.C.,Chang, W.R. (登録日: 2001-11-07, 公開日: 2003-11-04, 最終更新日: 2023-10-25)

主引用文献

Liu, S.Q.,Chang, T.,Liu, M.Y.,LeGall, J.,Chang, W.C.,Zhang, J.P.,Liang, D.C.,Chang, W.R.
Crystal structure of a NO-forming nitrite reductase mutant: an analog of a transition state in enzymatic reaction
Biochem.Biophys.Res.Commun., 302:568-574, 2003

PubMed Abstract: I257E was obtained by site directed mutagenesis of nitrite reductase from Achromobacter cycloclastes. The mutant has no enzyme activity. Its crystal structure determined at 1.65A resolution shows that the side-chain carboxyl group of the mutated residue, Glu257, coordinates with the type 2 copper in the mutant and blocks the contact between the type 2 copper and its solvent channel, indicating that the accessibility of the type 2 copper is essential for maintaining the activity of nitrite reductase. The carboxylate is an analog of the substrate, nitrite, but the distances between the type 2 copper and the two oxygen atoms of the side-chain carboxyl group are reversed in comparison to the binding of nitrite to the native enzyme. In the mutant, both the type 2 copper and the N epsilon atom on the imidazole ring of its coordinated residue His135 move in the substrate binding direction relative to the native enzyme. In addition, an EPR study showed that the type 2 copper in the mutant is in a reduced state. We propose that mutant I257E is in a state corresponding to a transition state in the enzymatic reaction.

PubMed: 12615072
DOI: 10.1016/S0006-291X(03)00166-9

実験手法

X-RAY DIFFRACTION (1.65 Å)