1KBM

SOLUTION STRUCTURE OF AN 11-MER DNA DUPLEX CONTAINING 6-THIOGUANINE OPPOSITE THYMINE

1KBM の概要

• エントリーDOI: 10.2210/pdb1kbm/pdb
• 関連するPDBエントリー: 1KB1
• 分子名称: 5'-D(*CP*GP*TP*AP*CP*(S6G)P*CP*AP*TP*GP*C)-3', 5'-D(*GP*CP*AP*TP*GP*TP*GP*TP*AP*CP*G)-3' (2 entities in total)
• 機能のキーワード: thioguanine, 6-thioguanine, tg, 6tg, s6g, thiopurine, double helix, b-form dna, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 6739.47

構造登録者

Bohon, J.,De Los Santos, C.R. (登録日: 2001-11-06, 公開日: 2001-11-28, 最終更新日: 2024-05-22)

主引用文献

Bohon, J.,de los Santos, C.R.
Structural effect of the anticancer agent 6-thioguanine on duplex DNA.
Nucleic Acids Res., 31:1331-1338, 2003

PubMed Abstract: The incorporation of 6-thioguanine (S6G) into DNA is an essential step in the cytotoxic activity of thiopurines. However, the structural effects of this substitution on duplex DNA have not been fully characterized. Here, we present the solution structures of DNA duplexes containing S6G opposite thymine (S6G.T) and opposite cytosine (S6G.C), solved by high-resolution NMR spectroscopy and restrained molecular dynamics. The data indicate that both duplexes adopt right-handed helical conformations with all Watson-Crick hydrogen bonding in place. The S6G.T structures exhibit a wobble-type base pairing at the lesion site, with thymine shifted toward the major groove and S6G displaced toward the minor groove. Aside from the lesion site, the helices, including the flanking base pairs, are not highly perturbed by the presence of the lesion. Surprisingly, thermal dependence experiments suggest greater stability in the S6G-T mismatch than the S6G-C base pair.

PubMed: 12582253
DOI: 10.1093/nar/gkg203

実験手法

SOLUTION NMR