1KAW
STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB)
Summary for 1KAW
| Entry DOI | 10.2210/pdb1kaw/pdb |
| Descriptor | SINGLE-STRANDED DNA BINDING PROTEIN (2 entities in total) |
| Functional Keywords | dna-binding protein, single stranded dna binding protein, ssb, dna binding protein |
| Biological source | Escherichia coli |
| Total number of polymer chains | 4 |
| Total formula weight | 57920.62 |
| Authors | Raghunathan, S.,Waksman, G. (deposition date: 1996-12-06, release date: 1997-12-31, Last modification date: 2024-02-07) |
| Primary citation | Raghunathan, S.,Ricard, C.S.,Lohman, T.M.,Waksman, G. Crystal structure of the homo-tetrameric DNA binding domain of Escherichia coli single-stranded DNA-binding protein determined by multiwavelength x-ray diffraction on the selenomethionyl protein at 2.9-A resolution. Proc.Natl.Acad.Sci.USA, 94:6652-6657, 1997 Cited by PubMed Abstract: The crystal structure of the tetrameric DNA-binding domain of the single-stranded DNA binding protein from Escherichia coli was determined at a resolution of 2.9 A using multiwavelength anomalous dispersion. Each monomer in the tetramer is topologically similar to an oligomer-binding fold. Two monomers each contribute three beta-strands to a single six-stranded beta-sheet to form a dimer. Two dimer-dimer interfaces are observed within the crystal. One of these stabilizes the tetramer in solution. The other interface promotes a superhelical structure within the crystal that may reflect tetramer-tetramer interactions involved in the positive cooperative binding of the single-stranded DNA-binding protein to single-stranded DNA. PubMed: 9192620DOI: 10.1073/pnas.94.13.6652 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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