1KA1

The PAPase Hal2p complexed with calcium and magnesium ions and reaction substrate: PAP

1KA1 の概要

• エントリーDOI: 10.2210/pdb1ka1/pdb
• 関連するPDBエントリー: 1QGX 1k9y 1k9z 1ka0
• 分子名称: Halotolerance protein HAL2, CALCIUM ION, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: nucleotidase, salt tolerance, inositol, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Cytoplasm: P32179
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39768.92

構造登録者

Patel, S.,Albert, A.,Blundell, T.L. (登録日: 2001-10-31, 公開日: 2001-11-07, 最終更新日: 2023-08-16)

主引用文献

Patel, S.,Martinez-Ripoll, M.,Blundell, T.L.,Albert, A.
Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases.
J.Mol.Biol., 320:1087-1094, 2002

PubMed Abstract: Li(+)-sensitive/Mg(2+)-dependent phosphatases have attracted considerable attention since they have been proposed as targets for lithium therapy in the treatment of manic-depressive patients. The members of this enzyme superfamily display low levels of sequence identity while possessing a common fold and active site. Extensive structural and biochemical data demonstrate the direct involvement of two metal ions in catalysis, and show that lithium exerts its inhibitory action by blocking the products at the active site. By exploiting the different inhibitory properties of magnesium and calcium, we have been able to solve the X-ray structures of the Li(+)-sensitive/Mg(2+)-dependent 3'-phosphoadenosine-5'-phosphatase in complex with its substrate and with its products. The structural comparison of these complexes provides a 3D picture of the different stages of the catalytic cycle. This gives new insights into the understanding of the biological function of this group of enzymes and their lithium inhibition, and should assist in the design of improved inhibitors of therapeutic value.

PubMed: 12126627
DOI: 10.1016/S0022-2836(02)00564-8

実験手法

X-RAY DIFFRACTION (1.3 Å)