1K92

Crystal Structure of Uncomplexed E. coli Argininosuccinate Synthetase

1K92 の概要

• エントリーDOI: 10.2210/pdb1k92/pdb
• 関連するPDBエントリー: 1K97
• 分子名称: ARGININOSUCCINATE SYNTHASE, SULFATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: n-type atp pyrophosphatase, ligase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm (Probable): P0A6E4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 51539.67

構造登録者

Lemke, C.T.,Howell, P.L. (登録日: 2001-10-26, 公開日: 2001-12-07, 最終更新日: 2024-02-07)

主引用文献

Lemke, C.T.,Howell, P.L.
The 1.6 A crystal structure of E. coli argininosuccinate synthetase suggests a conformational change during catalysis.
Structure, 9:1153-1164, 2001

PubMed Abstract: Argininosuccinate synthetase (AS) is the rate-limiting enzyme of both the urea and arginine-citrulline cycles. In mammals, deficiency of AS leads to citrullinemia, a debilitating and often fatal autosomal recessive urea cycle disorder, whereas its overexpression for sustained nitric oxide production via the arginine-citrulline cycle leads to the potentially fatal hypotension associated with septic and cytokine-induced circulatory shock.

PubMed: 11738042
DOI: 10.1016/S0969-2126(01)00683-9

実験手法

X-RAY DIFFRACTION (1.6 Å)