1K8H

NMR Structure of Small Protein B (SmpB) from Aquifex aeolicus

1K8H の概要

• エントリーDOI: 10.2210/pdb1k8h/pdb
• 分子名称: Small protein B (1 entity in total)
• 機能のキーワード: smpb, ssra associated protein, rna binding protein
• 由来する生物種: Aquifex aeolicus
• 細胞内の位置: Cytoplasm (Potential): O66640
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15523.29

構造登録者

Dong, G.,Hoffman, D.W. (登録日: 2001-10-24, 公開日: 2002-03-20, 最終更新日: 2024-05-22)

主引用文献

Dong, G.,Nowakowski, J.,Hoffman, D.W.
Structure of small protein B: the protein component of the tmRNA-SmpB system for ribosome rescue.
EMBO J., 21:1845-1854, 2002

PubMed Abstract: Small protein B (SmpB) is an essential component of the highly conserved tmRNA-SmpB system that has the dual function of releasing stalled ribosomes from damaged messenger RNAs and targeting incompletely synthesized protein fragments for degradation. Nuclear magnetic resonance (NMR) analysis of SmpB from Aquifex aeolicus revealed an antiparallel beta-barrel structure, with three helices packed outside the core of the barrel. While the overall structure of SmpB appears to be unique, the structure does contain an embedded oligonucleotide binding fold; in this respect SmpB has similarity to several other RNA-binding proteins that are known to be associated with translation, including IF1, ribosomal protein S17 and the N-terminal domain of aspartyl tRNA synthetase. Conserved amino acids on the protein surface that are most likely to directly interact with the tmRNA were identified. The presence of widely separated clusters of conserved amino acids suggests that SmpB could function either by stabilizing two distal regions of the tmRNA, or by facilitating an interaction between the tmRNA and another component of the translational apparatus.

PubMed: 11927568
DOI: 10.1093/emboj/21.7.1845

実験手法

SOLUTION NMR