1K7A

Ets-1(331-440)+GGAG duplex

1K7A の概要

• エントリーDOI: 10.2210/pdb1k7a/pdb
• 関連するPDBエントリー: 1K78 1K79
• 分子名称: DNA (5'-D(*TP*AP*GP*TP*GP*CP*CP*GP*GP*AP*GP*AP*TP*GP*T)-3'), DNA (5'-D(*CP*AP*CP*AP*TP*CP*TP*CP*CP*GP*GP*CP*AP*CP*T)-3'), C-ets-1 Protein (3 entities in total)
• 機能のキーワード: ets domain, transcription factor, transcription-dna complex, transcription/dna
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P27577
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 44281.58

構造登録者

Garvie, C.W.,Hagman, J.,Wolberger, C. (登録日: 2001-10-18, 公開日: 2002-01-04, 最終更新日: 2024-02-07)

主引用文献

Garvie, C.W.,Hagman, J.,Wolberger, C.
Structural studies of Ets-1/Pax5 complex formation on DNA.
Mol.Cell, 8:1267-1276, 2001

PubMed Abstract: Pax5 regulates the B cell-specific expression of the mb-1 gene together with members of the Ets family of transcriptional activators. The Ets proteins on their own bind poorly to the Pax5/Ets binding site, but can be recruited to the site by cooperative interactions with Pax5. The structure of the ETS domain of Ets-1 and the paired domain of Pax5 bound to DNA reveals the molecular details of the selective recruitment of different Ets proteins by Pax5. Comparison with structures of Ets-1 alone bound to both high- and low-affinity DNA sites reveals that Pax5 alters the Ets-1 contacts with DNA. The ability of one protein to alter the DNA sequence-specific contacts of another provides a general mechanism for combinatorial regulation of transcription.

PubMed: 11779502
DOI: 10.1016/S1097-2765(01)00410-5

実験手法

X-RAY DIFFRACTION (2.8 Å)