1K6H

Solution Structure of Conserved AGNN Tetraloops: Insights into Rnt1p RNA processing

Summary for 1K6H

• Entry DOI: 10.2210/pdb1k6h/pdb
• Related: 1K6G
• Descriptor: RNA (5'-R(P*GP*GP*CP*GP*UP*GP*UP*UP*CP*AP*GP*AP*AP*GP*AP*AP*CP*GP*CP*GP*CP*C)-3') (1 entity in total)
• Functional Keywords: rna tetraloop, rna
• Total number of polymer chains: 1
• Total formula weight: 7112.31

Authors

Lebars, I.,Lamontagne, B.,Yoshizawa, S.,Abou Elela, S.,Fourmy, D. (deposition date: 2001-10-16, release date: 2001-12-19, Last modification date: 2024-05-22)

Primary citation

Lebars, I.,Lamontagne, B.,Yoshizawa, S.,Aboul-Elela, S.,Fourmy, D.
Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing.
EMBO J., 20:7250-7258, 2001

PubMed Abstract: Rnt1p, the yeast orthologue of RNase III, cleaves rRNAs, snRNAs and snoRNAs at a stem capped with conserved AGNN tetraloop. Here we show that 9 bp long stems ending with AGAA or AGUC tetraloops bind to Rnt1p and direct specific but sequence-independent RNA cleavage when provided with stems longer than 13 bp. The solution structures of these two tetraloops reveal a common fold for the terminal loop stabilized by non-canonical A-A or A-C pairs and extensive base stacking. The conserved nucleotides are stacked at the 5' side of the loop, exposing their Watson-Crick and Hoogsteen faces for recognition by Rnt1p. These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage.

PubMed: 11743001
DOI: 10.1093/emboj/20.24.7250

Experimental method

SOLUTION NMR