1K5J

The Crystal Structure of Nucleoplasmin-Core

1K5J の概要

• エントリーDOI: 10.2210/pdb1k5j/pdb
• 分子名称: Nucleoplasmin Core (2 entities in total)
• 機能のキーワード: beta-barrel, jellyroll, beta-bulge, pentamer, chaperone
• 由来する生物種: Xenopus laevis (African clawed frog)
• 細胞内の位置: Nucleus: P05221
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 68763.42

構造登録者

Dutta, S.,Akey, I.V.,Dingwall, C.,Hartman, K.L.,Laue, T.,Nolte, R.T.,Head, J.F.,Akey, C.W. (登録日: 2001-10-10, 公開日: 2001-11-01, 最終更新日: 2024-02-07)

主引用文献

Dutta, S.,Akey, I.V.,Dingwall, C.,Hartman, K.L.,Laue, T.,Nolte, R.T.,Head, J.F.,Akey, C.W.
The crystal structure of nucleoplasmin-core: implications for histone binding and nucleosome assembly.
Mol.Cell, 8:841-853, 2001

PubMed Abstract: The efficient assembly of histone complexes and nucleosomes requires the participation of molecular chaperones. Currently, there is a paucity of data on their mechanism of action. We now present the structure of an N-terminal domain of nucleoplasmin (Np-core) at 2.3 A resolution. The Np-core monomer is an eight-stranded beta barrel that fits snugly within a stable pentamer. In the crystal, two pentamers associate to form a decamer. We show that both Np and Np-core are competent to assemble large complexes that contain the four core histones. Further experiments and modeling suggest that these complexes each contain five histone octamers which dock to a central Np decamer. This work has important ramifications for models of histone storage, sperm chromatin decondensation, and nucleosome assembly.

PubMed: 11684019
DOI: 10.1016/S1097-2765(01)00354-9

実験手法

X-RAY DIFFRACTION (2.3 Å)