1K4A

STRUCTURE OF AGAA RNA TETRALOOP, NMR, 20 STRUCTURES

1K4A の概要

• エントリーDOI: 10.2210/pdb1k4a/pdb
• 関連するPDBエントリー: 1K4B
• 分子名称: 5'-R(*GP*GP*UP*UP*CP*AP*GP*AP*AP*GP*AP*AP*CP*C)-3' (1 entity in total)
• 機能のキーワード: ribonucleic acid, tetraloop, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4509.77

構造登録者

Wu, H.,Yang, P.K.,Butcher, S.E.,Kang, S.,Chanfreau, G.,Feigon, J. (登録日: 2001-10-07, 公開日: 2001-12-19, 最終更新日: 2024-05-22)

主引用文献

Wu, H.,Yang, P.K.,Butcher, S.E.,Kang, S.,Chanfreau, G.,Feigon, J.
A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III.
EMBO J., 20:7240-7249, 2001

PubMed Abstract: RNases III are a family of double-stranded RNA (dsRNA) endoribonucleases involved in the processing and decay of a large number of cellular RNAs as well as in RNA interference. The dsRNA substrates of Saccharomyces cerevisiae RNase III (Rnt1p) are capped by tetraloops with the consensus sequence AGNN, which act as the primary docking site for the RNase. We have solved the solution structures of two RNA hairpins capped by AGNN tetraloops, AGAA and AGUU, using NMR spectroscopy. Both tetraloops have the same overall structure, in which the backbone turn occurs on the 3' side of the syn G residue in the loop, with the first A and G in a 5' stack and the last two residues in a 3' stack. A non-bridging phosphate oxygen and the universal G which are essential for Rnt1p binding are strongly exposed. The compared biochemical and structural analysis of various tetraloop sequences defines a novel family of RNA tetraloop fold with the consensus (U/A)GNN and implicates this conserved structure as the primary determinant for specific recognition of Rnt1p substrates.

PubMed: 11743000
DOI: 10.1093/emboj/20.24.7240

実験手法

SOLUTION NMR