1K3K

Solution Structure of a Bcl-2 Homolog from Kaposi's Sarcoma Virus

Summary for 1K3K

• Entry DOI: 10.2210/pdb1k3k/pdb
• Descriptor: functional anti-apoptotic factor vBCL-2 homolog (1 entity in total)
• Functional Keywords: bcl-2, herpesvirus, solution structure, apoptosis
• Biological source: Human herpesvirus 8
• Total number of polymer chains: 1
• Total formula weight: 17758.22

Authors

Huang, Q.,Petros, A.M.,Virgin, H.W.,Fesik, S.W.,Olejniczak, E.T. (deposition date: 2001-10-03, release date: 2002-04-10, Last modification date: 2024-05-22)

Primary citation

Huang, Q.,Petros, A.M.,Virgin, H.W.,Fesik, S.W.,Olejniczak, E.T.
Solution structure of a Bcl-2 homolog from Kaposi sarcoma virus.
Proc.Natl.Acad.Sci.USA, 99:3428-3433, 2002

PubMed Abstract: Kaposi sarcoma-associated herpes virus (KSHV) contains a gene that has functional and sequence homology to the apoptotic Bcl-2 family of proteins [Sarid, R., Sato, T., Bohenzky, R. A., Russo, J. J. & Chang, Y. (1997) Nat. Med. 3, 293-298]. The viral Bcl-2 protein promotes survival of infected cells and may contribute to the development of Kaposi sarcoma tumors [Boshoff, C. & Chang, Y. (2001) Annu. Rev. Med. 52, 453-470]. Here we describe the solution structure of the viral Bcl-2 homolog from KSHV. Comparison of the KSHV Bcl-2 structure to that of Bcl-2 and Bcl-x(L) shows that although the overall fold is the same, there are key differences in the lengths of the helices and loops. Binding studies on peptides derived from the Bcl-2 homology region 3 of proapoptotic family members indicate that the specificity of the viral protein is very different from what was previously observed for Bcl-x(L) and Bcl-2, suggesting that the viral protein has evolved to have a different mechanism of action than the host proteins.

PubMed: 11904405
DOI: 10.1073/pnas.062525799

Experimental method

SOLUTION NMR