1K33

Crystal structure analysis of the gp41 core mutant

1K33 の概要

• エントリーDOI: 10.2210/pdb1k33/pdb
• 関連するPDBエントリー: 1K33
• 分子名称: Transmembrane glycoprotein GP41 (2 entities in total)
• 機能のキーワード: gp41, six-helix bundle, trimer-of-hairpins, membrane fusion, viral protein
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• 細胞内の位置: Transmembrane protein gp41: Virion membrane; Single-pass type I membrane protein. Surface protein gp120: Virion membrane; Peripheral membrane protein: P04578
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7837.67

構造登録者

Shu, W.,Lu, M. (登録日: 2001-10-01, 公開日: 2001-10-10, 最終更新日: 2024-02-07)

主引用文献

Wang, S.,York, J.,Shu, W.,Stoller, M.O.,Nunberg, J.H.,Lu, M.
Interhelical interactions in the gp41 core: implications for activation of HIV-1 membrane fusion.
Biochemistry, 41:7283-7292, 2002

PubMed Abstract: The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein complex (gp120-gp41) promotes viral entry by mediating the fusion of viral and cellular membranes. Formation of a stable trimer-of-hairpins structure in the gp41 ectodomain brings the two membranes into proximity, leading to membrane fusion. The core of this hairpin structure is a six-helix bundle in which three carboxyl-terminal outer helices pack against an inner trimeric coiled coil. Here we investigate the role of these conserved interhelical interactions on the structure and function of both the envelope glycoprotein and the gp41 core. We have replaced each of the eight amino acids at the buried face of the carboxyl-terminal helix with a representative amino acid, alanine. Structural and physicochemical characterization of the alanine mutants shows that hydrophobic interactions are a dominant factor in the stabilization of the six-helix bundle. Alanine substitutions at the Trp628, Trp631, Ile635, and Ile642 residues also affected envelope processing and/or gp120-gp41 association and abrogated the ability of the envelope glycoprotein to mediate cell-cell fusion. These results suggest that the amino-terminal region of the gp41 outer-layer alpha-helix plays a key role in the sequence of events associated with HIV-1 entry and have implications for the development of antibodies and small-molecule inhibitors of this conserved element.

PubMed: 12044159
DOI: 10.1021/bi025648y

実験手法

X-RAY DIFFRACTION (1.75 Å)