1K2K

NMR MINIMIZED AVERAGE STRUCTURE OF d(CGTACG)2

1K2K の概要

• エントリーDOI: 10.2210/pdb1k2k/pdb
• 関連するPDBエントリー: 1K2J
• NMR情報: BMRB: 5339
• 分子名称: 5'-D(*CP*GP*TP*AP*CP*G)-3' (1 entity in total)
• 機能のキーワード: self-complementary dna, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3618.43

構造登録者

Lam, S.L.,Ip, L.N. (登録日: 2001-09-28, 公開日: 2002-04-10, 最終更新日: 2024-05-22)

主引用文献

Lam, S.L.,Ip, L.N.
Low temperature solution structures and base pair stacking of double helical d(CGTACG)(2).
J.Biomol.Struct.Dyn., 19:907-917, 2002

PubMed Abstract: Solution structures and base pair stacking of a self- complementary DNA hexamer d(CGTACG)(2) have been studied at 5, 10 and 15 degrees C, respectively. The stacking interactions among the center base pair steps of the DNA duplex are found to improve when the terminal base pairs became less stable due to end fraying. A new structural quantity, the stacking sum (Sigma(s)), is introduced to indicate small changes in the stacking overlaps between base pairs. The improvements in the stacking overlaps to maintain the double helical conformation are probably the cause for the observed temperature dependent structural changes in double helical DNA molecule. A detailed analysis of the helical parameters, backbone torsion angles, base orientations and sugar conformations of these structures has been performed.

PubMed: 11922844

実験手法

SOLUTION NMR