1K2A

Modified Form of Eosinophil-derived Neurotoxin

1K2A の概要

• エントリーDOI: 10.2210/pdb1k2a/pdb
• 分子名称: eosinophil-derived neurotoxin, SULFATE ION (3 entities in total)
• 機能のキーワード: rnase a folding, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Lysosome (Probable): P10153
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15909.96

構造登録者

Chang, C.,Newton, D.L.,Rybak, S.M.,Wlodawer, A. (登録日: 2001-09-26, 公開日: 2002-04-03, 最終更新日: 2024-11-06)

主引用文献

Chang, C.,Newton, D.L.,Rybak, S.M.,Wlodawer, A.
Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities.
J.Mol.Biol., 317:119-130, 2002

PubMed Abstract: The crystal structure of a post-translationally modified form of eosinophil-derived neurotoxin (EDN) with four extra residues on its N terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A). Two of the extra residues can be placed unambiguously, while the density corresponding to two others is poor. The modified N terminus appears to influence the position of the catalytically important His129, possibly explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell line and other endothelial cell lines. Analysis of the structure and function suggests that the reason for cytotoxicity is most likely due to cellular recognition by the N-terminal extension, since the intrinsic activity of the enzyme is not sufficient for cytotoxicity and the N-terminal extension does not affect the conformation of EDN.

PubMed: 11916383
DOI: 10.1006/jmbi.2002.5406

実験手法

X-RAY DIFFRACTION (1 Å)