1K0S

Solution structure of the chemotaxis protein CheW from the thermophilic organism Thermotoga maritima

1K0S の概要

• エントリーDOI: 10.2210/pdb1k0s/pdb
• 分子名称: CHEMOTAXIS PROTEIN CHEW (1 entity in total)
• 機能のキーワード: chew, signaling protein
• 由来する生物種: Thermotoga maritima
• 細胞内の位置: Cytoplasm (By similarity): Q56311
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16977.84

構造登録者

Griswold, I.J.,Zhou, H.,Swanson, R.V.,Simon, M.I.,Dahlquist, F.W. (登録日: 2001-09-20, 公開日: 2002-02-06, 最終更新日: 2024-05-22)

主引用文献

Griswold, I.J.,Zhou, H.,Matison, M.,Swanson, R.V.,McIntosh, L.P.,Simon, M.I.,Dahlquist, F.W.
The solution structure and interactions of CheW from Thermotoga maritima.
Nat.Struct.Biol., 9:121-125, 2002

PubMed Abstract: Using protein from the hyperthermophile Thermotoga maritima, we have determined the solution structure of CheW, an essential component in the formation of the bacterial chemotaxis signaling complex. The overall fold is similar to the regulatory domain of the chemotaxis kinase CheA. In addition, interactions of CheW with CheA were monitored by nuclear magnetic resonance (NMR) techniques. The chemical shift perturbation data show the probable contacts that CheW makes with CheA. In combination with previous genetic data, the structure also suggests a possible binding site for the chemotaxis receptor. These results provide a structural basis for a model in which CheW acts as a molecular bridge between CheA and the cytoplasmic tails of the receptor.

PubMed: 11799399
DOI: 10.1038/nsb753

実験手法

SOLUTION NMR