1K09

Solution structure of BetaCore, A Designed Water Soluble Four-Stranded Antiparallel b-sheet Protein

1K09 の概要

• エントリーDOI: 10.2210/pdb1k09/pdb
• NMR情報: BMRB: 5183
• 分子名称: Core Module I, Core Module II, (7E)-4,9-dioxo-6-oxa-3,7,10-triazadodec-7-ene-1,12-dioic acid (3 entities in total)
• 機能のキーワード: four-stranded antiparallel beta-sheet, de novo protein
• 由来する生物種: Bos taurus (Bovine); 詳細
• 細胞内の位置: Secreted: P00974 P00974
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 5967.91

構造登録者

Carulla, N.,Woodward, C.,Barany, G. (登録日: 2001-09-18, 公開日: 2002-07-10, 最終更新日: 2024-02-21)

主引用文献

Carulla, N.,Woodward, C.,Barany, G.
BetaCore, a designed water soluble four-stranded antiparallel beta-sheet protein.
Protein Sci., 11:1539-1551, 2002

PubMed Abstract: BetaCore is a designed approximately 50-residue protein in which two BPTI-derived core modules, CM I and CM II, are connected by a 22-atom cross-link. At low temperature and pH 3, homo- and heteronuclear NMR data report a dominant folded ('f') conformation with well-dispersed chemical shifts, i, i+1 periodicity, numerous long-range NOEs, and slowed amide hydrogen isotope exchange patterns that is a four-stranded antiparallel beta-sheet with nonsymmetrical and specific association of CM I and CM II. BetaCore 'f' conformations undergo reversible, global, moderately cooperative, non-two-state thermal transitions to an equilibrium ensemble of unfolded 'u' conformations. There is a significant energy barrier between 'f' and 'u' conformations. This is the first designed four-stranded antiparallel beta-sheet that folds in water.

PubMed: 12021452
DOI: 10.1110/ps.4440102

実験手法

SOLUTION NMR