1JYI

CONCANAVALIN A/12-MER PEPTIDE COMPLEX

Summary for 1JYI

• Entry DOI: 10.2210/pdb1jyi/pdb
• Related: 1JOJ 1JUI 1JYC
• Descriptor: Concanavalin-Br, 12-mer peptide, MANGANESE (II) ION, ... (5 entities in total)
• Functional Keywords: lectin, sugar binding protein
• Biological source: Canavalia ensiformis (jack bean); More
• Total number of polymer chains: 8
• Total formula weight: 108331.38

Authors

Jain, D.,Kaur, K.J.,Sundaravadivel, B.,Salunke, D.M. (deposition date: 2001-09-12, release date: 2002-09-12, Last modification date: 2023-08-16)

Primary citation

Jain, D.,Kaur, K.J.,Sundaravadivel, B.,Salunke, D.M.
Structural and Functional Consequences of Peptide-carbohydrate Mimicry. Crystal Structure of a Carbohydrate-mimicking Peptide Bound to Concanavalin A.
J.Biol.Chem., 275:16098-16102, 2000

PubMed Abstract: The functional consequences of peptide-carbohydrate mimicry were analyzed on the basis of the crystal structure of concanavalin A (ConA) in complex with a carbohydrate-mimicking peptide, DVFYPYPYASGS. The peptide binds to the non-crystallographically related monomers of two independent dimers of ConA in two different modes, in slightly different conformations, demonstrating structural adaptability in ConA-peptide recognition. In one mode, the peptide has maximum interactions with ConA, and in the other, it shows relatively fewer contacts within this site but significant contacts with the symmetry-related subunit. Neither of the peptide binding sites overlaps with the structurally characterized mannose and trimannose binding sites on ConA. Despite this, the functional mimicry between the peptide and carbohydrate ligands was evident. The peptide-inhibited ConA induced T cell proliferation in a dose-dependent manner. The effect of the designed analogs of the peptide on ConA-induced T cell proliferation and their recognition by the antibody response against alpha-d-mannopyranoside indicate a role for aromatic residues in functional mimicry. Although the functional mimicry was observed between the peptide and carbohydrate moieties, the crystal structure of the ConA-peptide complex revealed that the two peptide binding sites are independent of the methyl alpha-d-mannopyranoside binding site.

PubMed: 10821862
DOI: 10.1074/jbc.275.21.16098

Experimental method

X-RAY DIFFRACTION (2.75 Å)