1JY6

B4DIMERA: A DE NOVO DESIGNED FOUR-STRANDED BETA-SHEET ASSEMBLED USING A DISULFIDE BOND

1JY6 の概要

• エントリーDOI: 10.2210/pdb1jy6/pdb
• 関連するPDBエントリー: 1JY4
• 分子名称: B4DIMER (1 entity in total)
• 機能のキーワード: four-stranded beta-sheet, disulfide bond, de novo protein design, de novo protein
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7875.27

構造登録者

Venkatraman, J.,Nagana Gowda, G.A.,Balaram, P. (登録日: 2001-09-11, 公開日: 2002-06-12, 最終更新日: 2024-10-30)

主引用文献

Venkatraman, J.,Nagana Gowda, G.A.,Balaram, P.
Design and construction of an open multistranded beta-sheet polypeptide stabilized by a disulfide bridge.
J.Am.Chem.Soc., 124:4987-4994, 2002

PubMed Abstract: The design and characterization of an open eight-stranded beta-sheet in a synthetic, 2-fold symmetric 70-residue peptide is described. The design strategy involves the generation of a 35-residue four-stranded beta-sheet peptide in which successive hairpins are nucleated by appropriately positioned (D)Pro-Xxx sequences. Oxidative dimerization using a single Cys residue positioned at the center of the C-terminal strand results in a disulfide-bridged eight-stranded structure. Nuclear Overhauser effects firmly establish an eight-stranded beta-sheet in methanol. In water, the outer strands are frayed, but a well-defined four-stranded beta-sheet stabilized by a disulfide bridge and a hydrophobic cluster is determined from NMR data. Comparison of the precursor peptide with the disulfide-bridged dimer reveals considerable enhancement of beta-sheet content in the latter, suggesting that the disulfide cross-link is an effective strategy for the stabilization of beta-sheets.

PubMed: 11982362
DOI: 10.1021/ja0174276

実験手法

SOLUTION NMR