1JXC

Minimized NMR structure of ATT, an Arabidopsis trypsin/chymotrypsin inhibitor

1JXC の概要

• エントリーDOI: 10.2210/pdb1jxc/pdb
• 分子名称: Putative trypsin inhibitor ATTI-2 (1 entity in total)
• 機能のキーワード: att, trypsin inhibitor, chymotrypsin inhibitor, structural genomics, protein structure initiative, center for eukaryotic structural genomics, cesg, hydrolase inhibitor
• 由来する生物種: Arabidopsis thaliana (thale cress)
• 細胞内の位置: Secreted (By similarity): Q42328
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7451.47

構造登録者

Zhao, Q.,Chae, Y.K.,Markley, J.L.,Center for Eukaryotic Structural Genomics (CESG) (登録日: 2001-09-06, 公開日: 2003-01-07, 最終更新日: 2024-11-06)

主引用文献

Zhao, Q.,Chae, Y.K.,Markley, J.L.
NMR Solution Structure of ATTp, an Arabidopsis thaliana Trypsin Inhibitor
Biochemistry, 41:12284-12296, 2002

PubMed Abstract: The three-dimensional structure of the precursor form of the Arabidopsis thaliana trypsin inhibitor (ATT(p), GenBank entry Z46816), a 68-residue (approximately 7.5 kDa) rapeseed class proteinase inhibitor, has been determined in solution at pH 5.0 and 25 degrees C by multinuclear magnetic resonance spectroscopy. The protein contains one alpha-helix and two strands of antiparallel beta-sheet, with a type IV beta-turn connecting the two strands. The alpha-helix and the inhibitory loop are connected to the beta-sheet through three disulfide bridges; a fourth disulfide bridge connects the N- and C-termini. The overall structural topology of ATT(p) is similar to those of the sweet tasting protein brazzein (rmsd of 3.0 A) and the antifungal protein Rs-Afp1 [a knottin protein from radish (Raphanus sativus), rmsd of 2.7 A]. The precursor segment in ATT(p) is disordered, as visualized by the final 20-conformer ensemble and as confirmed by (15)N heteronuclear NOE analysis. The overall fold of ATT(p) is distinct from those of other classes of serine proteinase inhibitors except in the inhibitor loop; therefore, it represents a new inhibitor fold.

PubMed: 12369816
DOI: 10.1021/bi025702a

実験手法

SOLUTION NMR