1JWC

NMR SOLUTION STRUCTURE OF THE RNA HAIRPIN BINDING SITE FOR THE HISTONE STEM-LOOP BINDING PROTEIN

1JWC の概要

• エントリーDOI: 10.2210/pdb1jwc/pdb
• 関連するPDBエントリー: 1JU7
• NMR情報: BMRB: 5170
• 分子名称: 5'-R(*GP*GP*CP*CP*AP*AP*AP*GP*GP*CP*CP*CP*UP*UP*UP*UP*CP*AP*GP*GP*GP*CP*CP*AP*CP*CP*CP*A)-3' (1 entity in total)
• 機能のキーワード: hairpin, tetraloop, 3' stack, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8928.38

構造登録者

Dejong, E.S.,Marzluff, W.F.,Nikonowicz, E.P. (登録日: 2001-09-03, 公開日: 2002-03-20, 最終更新日: 2024-05-22)

主引用文献

DeJong, E.S.,Marzluff, W.F.,Nikonowicz, E.P.
NMR structure and dynamics of the RNA-binding site for the histone mRNA stem-loop binding protein.
RNA, 8:83-96, 2002

PubMed Abstract: The 3' end of replication-dependent histone mRNAs terminate in a conserved sequence containing a stem-loop. This 26-nt sequence is the binding site for a protein, stem-loop binding protein (SLBP), that is involved in multiple aspects of histone mRNA metabolism and regulation. We have determined the structure of the 26-nt sequence by multidimensional NMR spectroscopy. There is a 16-nt stem-loop motif, with a conserved 6-bp stem and a 4-nt loop. The loop is closed by a conserved U.A base pair that terminates the canonical A-form stem. The pyrimidine-rich 4-nt loop, UUUC, is well organized with the three uridines stacking on the helix, and the fourth base extending across the major groove into the solvent. The flanking nucleotides at the base of the hairpin stem do not assume a unique conformation, despite the fact that the 5' flanking nucleotides are a critical component of the SLBP binding site.

PubMed: 11871662
DOI: 10.1017/S1355838202013869

実験手法

SOLUTION NMR