1JWB

Structure of the Covalent Acyl-Adenylate Form of the MoeB-MoaD Protein Complex

Summary for 1JWB

• Entry DOI: 10.2210/pdb1jwb/pdb
• Related: 1FMO 1JW9 1JWA
• Descriptor: MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN, MOLYBDOPTERIN [MPT] CONVERTING FACTOR, SUBUNIT 1, ZINC ION, ... (6 entities in total)
• Functional Keywords: moeb: modified rossmann fold; (2) cys-x-x-cys zinc-binding motifs; moad: ubiquitin-like fold, ligase
• Biological source: Escherichia coli; More
• Total number of polymer chains: 2
• Total formula weight: 36015.36

Authors

Lake, M.W.,Wuebbens, M.M.,Rajagopalan, K.V.,Schindelin, H. (deposition date: 2001-09-03, release date: 2001-11-21, Last modification date: 2017-10-04)

Primary citation

Lake, M.W.,Wuebbens, M.M.,Rajagopalan, K.V.,Schindelin, H.
Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex.
Nature, 414:325-329, 2001

PubMed Abstract: The activation of ubiquitin and related protein modifiers is catalysed by members of the E1 enzyme family that use ATP for the covalent self-attachment of the modifiers to a conserved cysteine. The Escherichia coli proteins MoeB and MoaD are involved in molybdenum cofactor (Moco) biosynthesis, an evolutionarily conserved pathway. The MoeB- and E1-catalysed reactions are mechanistically similar, and despite a lack of sequence similarity, MoaD and ubiquitin display the same fold including a conserved carboxy-terminal Gly-Gly motif. Similar to the E1 enzymes, MoeB activates the C terminus of MoaD to form an acyl-adenylate. Subsequently, a sulphurtransferase converts the MoaD acyl-adenylate to a thiocarboxylate that acts as the sulphur donor during Moco biosynthesis. These findings suggest that ubiquitin and E1 are derived from two ancestral genes closely related to moaD and moeB. Here we present the crystal structures of the MoeB-MoaD complex in its apo, ATP-bound, and MoaD-adenylate forms, and highlight the functional similarities between the MoeB- and E1-substrate complexes. These structures provide a molecular framework for understanding the activation of ubiquitin, Rub, SUMO and the sulphur incorporation step during Moco and thiamine biosynthesis.

PubMed: 11713534
DOI: 10.1038/35104586

Experimental method

X-RAY DIFFRACTION (2.1 Å)