1JW2

SOLUTION STRUCTURE OF HEMOLYSIN EXPRESSION MODULATING PROTEIN Hha FROM ESCHERICHIA COLI. Ontario Centre for Structural Proteomics target EC0308_1_72; Northeast Structural Genomics Target ET88

Summary for 1JW2

• Entry DOI: 10.2210/pdb1jw2/pdb
• NMR Information: BMRB: 5166
• Descriptor: HEMOLYSIN EXPRESSION MODULATING PROTEIN Hha (1 entity in total)
• Functional Keywords: hha, hemolysin expression modulating protein, structural genomics, protein structure initiative, ocsp, nesg, psi, northeast structural genomics consortium, gene regulation
• Biological source: Escherichia coli
• Total number of polymer chains: 1
• Total formula weight: 8642.02

Authors

Chang, X.,Yee, A.,Savchenko, A.,Edwards, A.M.,Arrowsmith, C.H.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2001-09-02, release date: 2002-02-27, Last modification date: 2024-05-22)

Primary citation

Yee, A.,Chang, X.,Pineda-Lucena, A.,Wu, B.,Semesi, A.,Le, B.,Ramelot, T.,Lee, G.M.,Bhattacharyya, S.,Gutierrez, P.,Denisov, A.,Lee, C.H.,Cort, J.R.,Kozlov, G.,Liao, J.,Finak, G.,Chen, L.,Wishart, D.,Lee, W.,McIntosh, L.P.,Gehring, K.,Kennedy, M.A.,Edwards, A.M.,Arrowsmith, C.H.
An NMR approach to structural proteomics
Proc.Natl.Acad.Sci.USA, 99:1825-1830, 2002

PubMed Abstract: The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

PubMed: 11854485
DOI: 10.1073/pnas.042684599

Experimental method

SOLUTION NMR