1JVZ

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid

Summary for 1JVZ

• Entry DOI: 10.2210/pdb1jvz/pdb
• Related: 1JW0
• Descriptor: cephalosporin acylase alpha chain, cephalosporin acylase beta chain, 7BETA-(4CARBOXYBUTANAMIDO) CEPHALOSPORANIC ACID, ... (4 entities in total)
• Functional Keywords: cephalosporin acylase, glutaryl-7-aminocephalosporanic acid, hydrolase
• Biological source: Brevundimonas diminuta; More
• Cellular location: Periplasm (Potential): Q9L5D6 Q9L5D6
• Total number of polymer chains: 2
• Total formula weight: 76544.77

Authors

Kim, Y.,Hol, W.G.J. (deposition date: 2001-09-01, release date: 2002-09-01, Last modification date: 2024-10-09)

Primary citation

Kim, Y.,Hol, W.G.
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity
CHEM.BIOL., 8:1253-1264, 2001

PubMed Abstract: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC.

PubMed: 11755403
DOI: 10.1016/S1074-5521(01)00092-8

Experimental method

X-RAY DIFFRACTION (2.6 Å)