1JVZ

Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid

1JVZ の概要

• エントリーDOI: 10.2210/pdb1jvz/pdb
• 関連するPDBエントリー: 1JW0
• 分子名称: cephalosporin acylase alpha chain, cephalosporin acylase beta chain, 7BETA-(4CARBOXYBUTANAMIDO) CEPHALOSPORANIC ACID, ... (4 entities in total)
• 機能のキーワード: cephalosporin acylase, glutaryl-7-aminocephalosporanic acid, hydrolase
• 由来する生物種: Brevundimonas diminuta; 詳細
• 細胞内の位置: Periplasm (Potential): Q9L5D6 Q9L5D6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 76544.77

構造登録者

Kim, Y.,Hol, W.G.J. (登録日: 2001-09-01, 公開日: 2002-09-01, 最終更新日: 2024-10-09)

主引用文献

Kim, Y.,Hol, W.G.
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity
CHEM.BIOL., 8:1253-1264, 2001

PubMed Abstract: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC.

PubMed: 11755403
DOI: 10.1016/S1074-5521(01)00092-8

実験手法

X-RAY DIFFRACTION (2.6 Å)