1JVQ

Crystal structure at 2.6A of the ternary complex between antithrombin, a P14-P8 reactive loop peptide, and an exogenous tetrapeptide

1JVQ の概要

• エントリーDOI: 10.2210/pdb1jvq/pdb
• 関連するPDBエントリー: 1BR8
• 分子名称: ANTITHROMBIN-III, P14-P8 reactive loop peptide, exogenous Cholecystokinin tetrapeptide, ... (6 entities in total)
• 機能のキーワード: loop-sheet polymer, beta-barrel, blood clotting, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted, extracellular space: P01008
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 101450.25

構造登録者

Zhou, A.,Huntington, J.A.,Lomas, D.A.,Carrell, R.W.,Stein, P.E. (登録日: 2001-08-31, 公開日: 2003-06-03, 最終更新日: 2024-11-13)

主引用文献

Zhou, A.,Stein, P.E.,Huntington, J.A.,Sivasothy, P.,Lomas, D.A.,Carrell, R.W.
How small peptides block and reverse serpin polymerisation
J.Mol.Biol., 342:931-941, 2004

PubMed Abstract: Many of the late-onset dementias, including Alzheimer's disease and the prion encephalopathies, arise from the aberrant aggregation of individual proteins. The serpin family of serine protease inhibitors provides a well-defined structural example of such pathological aggregation, as its mutant variants readily form long-chain polymers, resulting in diseases ranging from thrombosis to dementia. The intermolecular linkages result from the insertion of the reactive site loop of one serpin molecule into the middle strand (s4A) position of the A beta-sheet of another molecule. We define here the structural requirements for small peptides to competitively bind to and block the s4A position to prevent this intermolecular linkage and polymerisation. The entry and anchoring of blocking-peptides is facilitated by the presence of a threonine which inserts into the site equivalent to P8 of s4A. But the critical requirement for small blocking-peptides is demonstrated in crystallographic structures of the complexes formed with selected tri- and tetrapeptides. These structures indicate that the binding is primarily due to the insertion of peptide hydrophobic side-chains into the P4 and P6 sites of s4A. The findings allow the rational design of synthetic blocking-peptides small enough to be suitable for mimetic design. This is demonstrated here with a tetrapeptide that preferentially blocks the polymerisation of a pathologically unstable serpin commonly present in people of European descent.

PubMed: 15342247
DOI: 10.1016/j.jmb.2004.07.078

実験手法

X-RAY DIFFRACTION (2.6 Å)