1JS2

Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S] cluster

Summary for 1JS2

• Entry DOI: 10.2210/pdb1js2/pdb
• Descriptor: high-potential iron protein, IRON/SULFUR CLUSTER (3 entities in total)
• Functional Keywords: serine ligated [4fe-4s] cluster, electron transport
• Biological source: Allochromatium vinosum
• Cellular location: Periplasm: P00260
• Total number of polymer chains: 4
• Total formula weight: 39148.72

Authors

Mansy, S.S.,Xiong, Y.,Hemann, C.,Hille, R.,Sundaralingam, M.,Cowan, J.A. (deposition date: 2001-08-16, release date: 2002-01-25, Last modification date: 2024-02-07)

Primary citation

Mansy, S.S.,Xiong, Y.,Hemann, C.,Hille, R.,Sundaralingam, M.,Cowan, J.A.
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster.
Biochemistry, 41:1195-1201, 2002

PubMed Abstract: The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.

PubMed: 11802718
DOI: 10.1021/bi011811y

Experimental method

X-RAY DIFFRACTION (1.9 Å)