1JS2

Crystal structure of C77S HiPIP: a serine ligated [4Fe-4S] cluster

1JS2 の概要

• エントリーDOI: 10.2210/pdb1js2/pdb
• 分子名称: high-potential iron protein, IRON/SULFUR CLUSTER (3 entities in total)
• 機能のキーワード: serine ligated [4fe-4s] cluster, electron transport
• 由来する生物種: Allochromatium vinosum
• 細胞内の位置: Periplasm: P00260
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 39148.72

構造登録者

Mansy, S.S.,Xiong, Y.,Hemann, C.,Hille, R.,Sundaralingam, M.,Cowan, J.A. (登録日: 2001-08-16, 公開日: 2002-01-25, 最終更新日: 2024-02-07)

主引用文献

Mansy, S.S.,Xiong, Y.,Hemann, C.,Hille, R.,Sundaralingam, M.,Cowan, J.A.
Crystal structure and stability studies of C77S HiPIP: a serine ligated [4Fe-4S] cluster.
Biochemistry, 41:1195-1201, 2002

PubMed Abstract: The crystal structure of Chromatium vinosum C77S HiPIP has been determined and is compared with that of wild type. This is the first reported crystal structure of a Ser ligated [4Fe-4S] cluster and reveals a 0.11 A shortening of the Fe-O bond (relative to Fe-S), but only minor structural alterations of the overall tertiary structure. Coordination changes are corroborated by resonance Raman spectroscopy. Comparison of the crystal and solution structures for HiPIPs identifies Phe48 as the main controller of solvent access to the Fe-S cluster; however, there is no significant change in cluster solvation of the C77S mutant relative to WT HiPIP. Ser ligation ultimately results in decreased cluster stability due to increased sensitivity to proton mediated degradation.

PubMed: 11802718
DOI: 10.1021/bi011811y

実験手法

X-RAY DIFFRACTION (1.9 Å)