1JR9

Crystal Structure of manganese superoxide dismutases from Bacillus halodenitrificans

Summary for 1JR9

• Entry DOI: 10.2210/pdb1jr9/pdb
• Descriptor: manganese superoxide dismutase, MANGANESE (II) ION, ZINC ION, ... (4 entities in total)
• Functional Keywords: manganese superoxide dismutase, oxidoreductase
• Biological source: Virgibacillus halodenitrificans
• Cellular location: Cytoplasm: Q7SIC3
• Total number of polymer chains: 1
• Total formula weight: 22808.61

Authors

Liao, J.,Liu, M.Y.,Chang, T.,Li, M.,LeGall, J.,Gui, L.L.,Zhang, J.P.,Jiang, T.,Liang, D.C.,Chang, W.R. (deposition date: 2001-08-13, release date: 2002-08-14, Last modification date: 2023-10-25)

Primary citation

Liao, J.,Liu, M.Y.,Chang, T.,Li, M.,Le Gall, J.,Gui, L.L.,Zhang, J.P.,Jiang, T.,Liang, D.C.,Chang, W.R.
Three-dimensional structure of manganese superoxide dismutase from Bacillus halodenitrificans, a component of the so-called "green protein".
J.Struct.Biol., 139:171-180, 2002

PubMed Abstract: A so-called "green protein" has been purified from a moderate halophilic eubacterium, Bacillus halodenitrificans (ATCC 49067), under anaerobic conditions. The protein, which might play an important role in denitrification, dissociates mainly into two components after exposure to air: a manganese superoxide dismutase (GP-MnSOD) and a nucleoside diphosphate kinase. As a first step in elucidating the overall structure of the green protein and the role of each component, the 2.8-A resolution crystal structure of GP-MnSOD was determined. Compared with other manganese dismutases, GP-MnSOD shows two significant characteristics. The first is that the entrance to its substrate channel has an additional basic residue-Lys38. The second is that its surface is decorated with an excess of acidic over basic residues. All these structural features may be related to GP-MnSOD's high catalytic activity and its endurance against the special cytoplasm of B. halodenitrificans. The structure of GP-MnSOD provides the basis for recognizing its possible role and assembly state in the green protein.

PubMed: 12457847
DOI: 10.1016/S0006-291X(02)00436-9

Experimental method

X-RAY DIFFRACTION (2.8 Å)