1JQQ

Crystal structure of Pex13p(301-386) SH3 domain

1JQQ の概要

• エントリーDOI: 10.2210/pdb1jqq/pdb
• 分子名称: PEROXISOMAL MEMBRANE PROTEIN PAS20 (2 entities in total)
• 機能のキーワード: compact beta-barrel of five anti-parrallel beta-strands, protein transport, membrane protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Peroxisome membrane; Single-pass membrane protein: P80667
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 43129.14

構造登録者

Douangamath, A.,Mayans, O.,Barnett, P.,Distel, B.,Wilmanns, M. (登録日: 2001-08-08, 公開日: 2002-12-06, 最終更新日: 2024-02-07)

主引用文献

Douangamath, A.,Filipp, F.V.,Klein, A.T.J.,Barnett, P.,Zou, P.,Voorn-Brouwer, T.,Vega, M.C.,Mayans, O.,Sattler, M.,Distel, B.,Wilmanns, M.
Topography for Independent Binding of alpha-Helical and PPII-Helical Ligands to a Peroxisomal SH3 Domain
Mol.Cell, 10:1007-1017, 2002

PubMed Abstract: While the function of most small signaling domains is confined to binary ligand interactions, the peroxisomal Pex13p SH3 domain has the unique capacity of binding to two different ligands, Pex5p and Pex14p. We have used this domain as a model to decipher its structurally independent ligand binding sites. By the combined use of X-ray crystallography, NMR spectroscopy, and circular dichroism, we show that the two ligands bind in unrelated conformations to patches located at opposite surfaces of this SH3 domain. Mutations in the Pex13p SH3 domain that abolish interactions within the Pex13p-Pex5p interface specifically impair PTS1-dependent protein import into yeast peroxisomes.

PubMed: 12453410
DOI: 10.1016/S1097-2765(02)00749-9

実験手法

X-RAY DIFFRACTION (2.65 Å)