1JPN

GMPPNP Complex of SRP GTPase NG Domain

1JPN の概要

• エントリーDOI: 10.2210/pdb1jpn/pdb
• 関連するPDBエントリー: 1JPJ
• 分子名称: SIGNAL RECOGNITION PARTICLE PROTEIN, CALCIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER, ... (5 entities in total)
• 機能のキーワード: ffh, srp, gmppnp, signal recognition particle, srp54, ng domain, signaling protein
• 由来する生物種: Thermus aquaticus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 66309.77

構造登録者

Padmanabhan, S.,Freymann, D.M. (登録日: 2001-08-02, 公開日: 2002-02-02, 最終更新日: 2023-08-16)

主引用文献

Padmanabhan, S.,Freymann, D.M.
The conformation of bound GMPPNP suggests a mechanism for gating the active site of the SRP GTPase.
Structure, 9:859-867, 2001

PubMed Abstract: The signal recognition particle (SRP) is a phylogenetically conserved ribonucleoprotein that mediates cotranslational targeting of secreted and membrane proteins to the membrane. Targeting is regulated by GTP binding and hydrolysis events that require direct interaction between structurally homologous "NG" GTPase domains of the SRP signal recognition subunit and its membrane-associated receptor, SR alpha. Structures of both the apo and GDP bound NG domains of the prokaryotic SRP54 homolog, Ffh, and the prokaryotic receptor homolog, FtsY, have been determined. The structural basis for the GTP-dependent interaction between the two proteins, however, remains unknown.

PubMed: 11566135
DOI: 10.1016/S0969-2126(01)00641-4

実験手法

X-RAY DIFFRACTION (1.9 Å)