1JMC

SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420

1JMC の概要

• エントリーDOI: 10.2210/pdb1jmc/pdb
• NMR情報: BMRB: 5821,5822,5823
• 分子名称: DNA (5'-D(*CP*CP*CP*CP*CP*CP*CP*C)-3'), PROTEIN (REPLICATION PROTEIN A (RPA)) (3 entities in total)
• 機能のキーワード: human ssdna binding replication protein a(rpa), single stranded dna-binding protein, protein-ssdna complex, complex (dna-binding protein-dna), replication-dna complex, replication/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: P27694
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29785.45

構造登録者

Bochkarev, A.,Pfuetzner, R.,Edwards, A.,Frappier, L. (登録日: 1996-11-11, 公開日: 1997-10-16, 最終更新日: 2024-11-20)

主引用文献

Bochkarev, A.,Pfuetzner, R.A.,Edwards, A.M.,Frappier, L.
Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.
Nature, 385:176-181, 1997

PubMed Abstract: The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. The structures of four SSBs have been solved, but the molecular details of the interaction of SSBs with DNA remain speculative. We report here the crystal structure at 2.4 A resolution of the single-stranded-DNA-binding domain of human replication protein A (RPA) bound to DNA. Replication protein A is a heterotrimeric SSB that is highly conserved in eukaryotes. The largest subunit, RPA70, binds to single-stranded (ss)DNA and mediates interactions with many cellular and viral proteins. The DNA-binding domain, which lies in the middle of RPA70, comprises two structurally homologous subdomains oriented in tandem. The ssDNA lies in a channel that extends from one subdomain to the other. The structure of each RPA70 subdomain is similar to those of the bacteriophage SSBs, indicating that the mechanism of ssDNA-binding is conserved.

PubMed: 8990123
DOI: 10.1038/385176a0

実験手法

X-RAY DIFFRACTION (2.4 Å)