1JKR

Testing the Water-Mediated HIN Recombinase DNA Recognition by Systematic Mutations

1JKR の概要

• エントリーDOI: 10.2210/pdb1jkr/pdb
• 関連するPDBエントリー: 1HCR 1IJW 1JJ6 1JJ8 1JKO 1JKP 1JKQ
• 分子名称: 5'-D(*TP*GP*TP*TP*TP*TP*TP*GP*AP*CP*AP*AP*GP*A)-3', 5'-D(*AP*TP*CP*TP*TP*GP*TP*CP*AP*AP*AP*AP*AP*C)-3', DNA-INVERTASE HIN, ... (5 entities in total)
• 機能のキーワード: water-mediated recognition, protein-dna complex, hin recombinase, t11c mutant, dna binding protein-dna complex, dna binding protein/dna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 14726.83

構造登録者

Chiu, T.K.,Sohn, C.,Johnson, R.C.,Dickerson, R.E. (登録日: 2001-07-13, 公開日: 2002-02-22, 最終更新日: 2023-08-16)

主引用文献

Chiu, T.K.,Sohn, C.,Dickerson, R.E.,Johnson, R.C.
Testing water-mediated DNA recognition by the Hin recombinase.
EMBO J., 21:801-814, 2002

PubMed Abstract: The Hin recombinase specifically recognizes its DNA-binding site by means of both major and minor groove interactions. A previous X-ray structure, together with new structures of the Hin DNA-binding domain bound to a recombination half-site that were solved as part of the present study, have revealed that two ordered water molecules are present within the major groove interface. In this report, we test the importance of these waters directly by X-ray crystal structure analysis of complexes with four mutant DNA sequences. These structures, combined with their Hin-binding properties, provide strong support for the critical importance of one of the intermediate waters. A lesser but demonstrable role is ascribed to the second water molecule. The mutant structures also illustrate the prominent roles of thymine methyls both in stabilizing intermediate waters and in interfering with water or amino acid side chain interactions with DNA.

PubMed: 11847127
DOI: 10.1093/emboj/21.4.801

実験手法

X-RAY DIFFRACTION (2.28 Å)