1JJS

NMR Structure of IBiD, A Domain of CBP/p300

1JJS の概要

• エントリーDOI: 10.2210/pdb1jjs/pdb
• 分子名称: CREB-BINDING PROTEIN (1 entity in total)
• 機能のキーワード: transcription regulation, coactivator, transcription
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasm (By similarity): P45481
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5584.50

構造登録者

Lin, C.H.,Hare, B.J.,Wagner, G.,Harrison, S.C.,Maniatis, T.,Fraenkel, E. (登録日: 2001-07-09, 公開日: 2001-10-03, 最終更新日: 2024-05-22)

主引用文献

Lin, C.H.,Hare, B.J.,Wagner, G.,Harrison, S.C.,Maniatis, T.,Fraenkel, E.
A small domain of CBP/p300 binds diverse proteins: solution structure and functional studies.
Mol.Cell, 8:581-590, 2001

PubMed Abstract: The transcriptional coactivators CBP and p300 are critical regulators of metazoan gene expression. They associate with many different DNA-bound transcription factors through small, conserved domains. We have identified a compactly folded 46 residue domain in CBP and p300, the IRF-3 binding domain (IBiD), and we have determined its structure by NMR. It has a helical framework containing an apparently flexible polyglutamine loop that participates in ligand binding. Spectroscopic data indicate that induced folding accompanies association of IBiD with its partners, which exhibit no evident sequence similarities. We demonstrate the significance both in vitro and in vivo of interactions between IBiD and a number of diverse partners. Thus, IBiD is an important contributor to signal integration by CBP and p300.

PubMed: 11583620
DOI: 10.1016/S1097-2765(01)00333-1

実験手法

SOLUTION NMR